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Mapping of the gene...
Mapping of the gene network that regulates glycan clock of ageing
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Frkatović-Hodžić, Azra (author)
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- Mijakovac, Anika (author)
- Sveučilište u Zagrebu,University of Zagreb
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- Miškec, Karlo (author)
- Sveučilište u Zagrebu,University of Zagreb
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show more...
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- Nostaeva, Arina (author)
- Novosibirsk State University
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- Sharapov, Sodbo Z. (author)
- M. V. Lomonosov Moscow State University
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Landini, Arianna (author)
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Haller, Toomas (author)
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- Akker, Erik van den (author)
- Universiteit Leiden (UL),Leiden University (UL),Technische Universiteit Delft,Delft University of Technology (TU Delft)
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- Sharma, Sapna (author)
- Helmholtz Zentrum,Helmholtz Center
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- Cuadrat, Rafael R.C. (author)
- Helmholtz Zentrum,Helmholtz Center
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- Mangino, Massimo (author)
- King's College London,Guy's and St Thomas' NHS Foundation Trust
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- Li, Yong (author)
- Universitats Klinikum Freiburg und Medizinische Fakultat
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Keser, Toma (author)
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Rudman, Najda (author)
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Stambuk, Tamara (author)
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Pučić-Baković, Maja (author)
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Trbojević-Akmačić, Irena (author)
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Gudelj, Ivan (author)
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Štambuk, Jerko (author)
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Pribić, Tea (author)
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Radovani, Barbara (author)
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Tominac, Petra (author)
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Fischer, Krista (author)
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- Beekman, Marian (author)
- Universiteit Leiden (UL),Leiden University (UL)
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- Wuhrer, Manfred (author)
- Universiteit Leiden (UL),Leiden University (UL)
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- Gieger, Christian (author)
- Helmholtz Zentrum,Helmholtz Center
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- Schulze, Matthias B. (author)
- Universität Potsdam,University of Potsdam
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- Wittenbecher, Clemens, 1981 (author)
- Harvard School of Public Health, United States,Chalmers tekniska högskola,Chalmers University of Technology
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Polasek, Ozren (author)
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Hayward, Caroline (author)
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Wilson, James F. (author)
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- Spector, Tim D. (author)
- King's College London
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- Köttgen, Anna (author)
- Universitats Klinikum Freiburg und Medizinische Fakultat,Johns Hopkins University
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Vučković, Frano (author)
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- Aulchenko, Yurii S. (author)
- Russian Academy of Sciences,M. V. Lomonosov Moscow State University
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- Vojta, Aleksandar (author)
- Sveučilište u Zagrebu,University of Zagreb
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Krištić, Jasminka (author)
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Klarić, Lucija (author)
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- Zoldoš, Vlatka (author)
- Sveučilište u Zagrebu,University of Zagreb
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Lauc, Gordan (author)
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(creator_code:org_t)
- 2023
- 2023
- English.
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In: Aging. - 1945-4589. ; 15:24, s. 14509-14552
- Related links:
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https://research.cha... (primary) (free)
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https://research.cha...
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https://doi.org/10.1...
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Abstract
Subject headings
Close
- Glycans are an essential structural component of immunoglobulin G (IgG) that modulate its structure and function. However, regulatory mechanisms behind this complex posttranslational modification are not well known. Previous genome-wide association studies (GWAS) identified 29 genomic regions involved in regulation of IgG glycosylation, but only a few were functionally validated. One of the key functional features of IgG glycosylation is the addition of galactose (galactosylation), a trait which was shown to be associated with ageing. We performed GWAS of IgG galactosylation (N=13,705) and identified 16 significantly associated loci, indicating that IgG galactosylation is regulated by a complex network of genes that extends beyond the galactosyltransferase enzyme that adds galactose to IgG glycans. Gene prioritization identified 37 candidate genes. Using a recently developed CRISPR/dCas9 system we manipulated gene expression of candidate genes in the in vitro IgG expression system. Upregulation of three genes, EEF1A1, MANBA and TNFRSF13B, changed the IgG glycome composition, which confirmed that these three genes are involved in IgG galactosylation in this in vitro expression system.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medical Genetics (hsv//eng)
Keyword
- CRISPR/dCas9
- glycan clock
- glycosylation
- genome-wide association study
- immunoglobulin G
Publication and Content Type
- art (subject category)
- ref (subject category)
Find in a library
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Aging
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To the university's database
- By the author/editor
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Frkatović-Hodžić ...
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Mijakovac, Anika
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Miškec, Karlo
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Nostaeva, Arina
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Sharapov, Sodbo ...
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Landini, Arianna
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show more...
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Haller, Toomas
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Akker, Erik van ...
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Sharma, Sapna
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Cuadrat, Rafael ...
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Mangino, Massimo
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Li, Yong
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Keser, Toma
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Rudman, Najda
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Stambuk, Tamara
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Pučić-Baković, M ...
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Trbojević-Akmači ...
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Gudelj, Ivan
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Štambuk, Jerko
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Pribić, Tea
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Radovani, Barbar ...
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Tominac, Petra
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Fischer, Krista
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Beekman, Marian
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Wuhrer, Manfred
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Gieger, Christia ...
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Schulze, Matthia ...
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Wittenbecher, Cl ...
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Polasek, Ozren
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Hayward, Carolin ...
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Wilson, James F.
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Spector, Tim D.
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Köttgen, Anna
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Vučković, Frano
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Aulchenko, Yurii ...
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Vojta, Aleksanda ...
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Krištić, Jasmink ...
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Klarić, Lucija
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Zoldoš, Vlatka
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Lauc, Gordan
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Medical Genetics
- Articles in the publication
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Aging
- By the university
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Chalmers University of Technology